Using the floating leaf disk technique, students will be able to investigate the effect the factors of their choice will have on the rate of photosynthesis they will set a control group that has no factors or variables and they will set an experimental group(s) that include the variables of their choice. Investigating photosynthesis with a floating leaf disk assay 2015 the time required for a leaf disk to float is an index of the net rate of photosynthesis in that leaf disk however, since some leaf disks will be early floaters and others will be late investigation of photosynthesis using the floating leaf disk assay. This activity will allow students to measure the rate at which the photosynthesis process occurs students will work in small groups to design an experiment with one independent variable and test this variable on spinach leaf disks. Using the floating disk assay, your problem is to design and carry out an experiment to test the effect of a variable on the rate of photosynthesis you chose which variable you will investigate. The problem with et50 is that it goes down as the rate of photosynthesis goes up- -it is an inverse relationship and creates the following type of graph (data from steucek, et al 1985).
In actuality, density of the leaf disk, venation, and concentration of the solution may play a role in disk buoyancy, not just photosynthetic rate 1 identify a testable variable that may influence the rate of photosynthesis. Photosynthesis investigation1 part 1 measuring the rate of photosynthesis you will use the floating leaf disk method to measure the rate of photosynthesis to begin, cut several disks from a spinach leaf and put these leaf disks in a cup of water 1 do your leaf disks float. An overview of a photosynthesis lab for agriscience, waterford union high school, wisconsin.
The rate of photosynthesis actually seemed to be picking up around the 9th and 10th leaf, this could mean another experiment with more than 10 leaves should follow to see how much higher the rate will increase and when it will finally start declining. To find out if light affects the rate of photosynthesis through an investigation with leaf discs of a spinach leaf hypothesis: if the light source is removed or becomes limited, then it will take much longer for the leaf disc to rise as cellular respiration will no longer be taking place as part of the equation for photosynthesis will be. Floating leaf disk assay for investigating photosynthesis pre-lab questions: answer these in your lab notebook before starting the experiment name at least four parameters that will affect the rate of photosynthesis. Photosynthesis in leaf disk introduction: photosynthesis is a process in which plants convert light energy (sunlight) into usable chemical energy create hypotheses about the effects of environmental variables on the rate of photosynthesis materials: 15 g sodium bicarbonate (baking soda) photosynthesis in leaf disks _____name 4. As the photosynthesis occurs, oxygen is released into the interior of the leaf, changing the leaf’s buoyancy, causing the disks to rise cellular respiration takes place at the same time, taking in the oxygen, and the rate of the disks rise at an indirect measurement of the net rate of photosynthesis.
The floating leaf disk assay for investigating photosynthesis (a resource page) brad williamson introduction: trying to find a good, quantitative procedure that students can use for exploring photosynthesis is a challenge. The floating leaf disk assay for investigating photosynthesis the biology behind the procedure: leaf disks float, normally when the air spaces are infiltrated with solution the overall density of the leaf. When you use a browser, like chrome, it saves some information from websites in its cache and cookies clearing them fixes certain problems, like loading or formatting issues on sites. This photosynthesis investigation includes two parts • part 1 measuring the rate of photosynthesis in this section, students learn how to use the floating leaf disk method to measure the rate of net photosynthesis (ie the rate of photosynthesis minus the rate of cellular respiration) they. As photosynthesis proceeds oxygen is released into the interior of the leaf which changes the buoyancy--causing the disks to rise since cellular respiration is taking place at the same time, consuming oxygen, the rate that the disks rises is an indirect measurement of the net rate of photosynthesis.
Lab #2 investigation 5: photosynthesis purpose the purpose of this lab is to measure the rate of photosynthesis using the floating leaf disk technique hypothesis if the bicarbonate is added, then the rate of photosynthesis will increase materials 1. Floating leaf disk photosynthesis lab in this lab, you will be using leaf disks, to assay the net rate of photosynthesis under various lighting conditions leaf disks normally float, however when the air spaces are infiltrated with carbon dioxide, the floating leaf disk assay. The floating leaf disk assay for investigating photosynthesis from brad williamson introduction: trying to find a good, quantitative procedure that students can use for exploring photosynthesis is a challenge.
Leaf disk assay teacher’s manual the rate of photosynthesis should correlate with the intensity of the light provided 10 according to your data, does light intensity affect the rate of photosynthesis explain results may vary as photosynthetic rates increase, more oxygen will be released. Investigating photosynthesis using a floating leaf disk assay introduction photosynthesis is the metabolic process used by all autotrophs to capture light energy and convert it to the chemical energy in carbohydrates.
Floating leaf disk photosynthesis lab when the air spaces are infiltrated with a solution the overall density of the leaf disk generated by photosynthesis, the rate that the disks rise is an indirect measurement of the net rate of photosynthesis in this lab,. Investigating the rate of photosynthesis by using leaf disc introduction- background theory photosynthesis is a process of converting light energy (trapped by chlorophyll) to chemical energy, and stores it in bonds of sugar. Using the floating leaf disk assay protocol as a tool to measure the rate of photosynthesis, design an experiment to investigate how varying an environmental or plant condition effects the rate of photosynthesis. Observations are made as the disk cells undergo photosynthesis and students will record the time it takes for leaf disks to rise to the surface as an extension you may propose that the floating disks be placed in the dark to determine if the disks.